Coding

Part:BBa_K1129020:Design

Designed by: UBC iGEM 2013   Group: iGEM13_British_Columbia   (2013-08-30)


Complete caffeine synthesis pathway under arabinose-induced promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1586
    Illegal NheI site found at 3065
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2595
    Illegal BglII site found at 4092
    Illegal BglII site found at 4188
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1526
    Illegal BamHI site found at 3005
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have yet to test for expression, and we may find that codon optimization is needed.


Source

The caffeine biosynthesis genes we assembled were from the 2013 Distribution Kit and were submitted by the 2012 TU Munich team: parts BBa_K801070, BBa_K801071, BBa_K801072.

References